Development of genomic SSR and potential EST-SSR markers in Bupleurum chinense DC.
نویسندگان
چکیده
منابع مشابه
Development and Characterization of Polymorphic EST-SSR and Genomic SSR Markers for Tibetan Annual Wild Barley
Tibetan annual wild barley is rich in genetic variation. This study was aimed at the exploitation of new SSRs for the genetic diversity and phylogenetic analysis of wild barley by data mining. We developed 49 novel EST-SSRs and confirmed 20 genomic SSRs for 80 Tibetan annual wild barley and 16 cultivated barley accessions. A total of 213 alleles were generated from 69 loci with an average of 3....
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Radish (Raphanus sativus L.) belongs to Brassicaceae family and is a close relative of Brassica. This species shows a wide morphological diversity, and is an important vegetable especially in Asia. However, molecular research of radish is behind compared to that of Brassica. For example, reports on SSR (simple sequence repeat) markers are limited. Here, we designed 417 radish SSR markers from S...
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Although Japanese morning glory (Ipomoea nil (L.) Roth.) has been used intensively for genetic studies, DNA markers have not been developed in Ipomoea nil sufficient to cover all chromosomes. Therefore, we conducted microsatellite (simple sequence repeats, SSR) marker development in I. nil for future genetic studies. From 92,662 expressed sequence tag (EST) sequences, 514 unique microsatellite-...
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S. N. Qureshi, Department of Plant and Soil Science, P.O. Box 9555, Mississippi State University, Mississippi State, MS 39762; S. Saha and J. N. Jenkins, USDA-ARS, Crop Science Research Laboratory, P.O. Box 5367, Mississippi State, MS 39762; Ramesh V. Kantety, Department of Crop & Soil Sciences, 726 Bradfield Hall, Cornell University, Ithaca, NY 14853 * Corresponding author: [email protected]...
متن کاملGenetic Purity Assessment of Castor Hybrids Using Est-ssr Markers
Maintenance of genetic purity of a hybrid is essential to exploit its full potential. Genetic purity is generally assessed by conducting grow out test (GOT) where morphological and floral characters are analyzed, however results may be influenced by the environment. Further, it is expensive and time consuming. DNA based markers are rapid, reliable and cost effective for hybrid purity assessment...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2009
ISSN: 1684-5315
DOI: 10.5897/ajb09.1385